In this sub-project we want to analyze the important contribution of the macrophage PI3K signaling pathway to metabolic health in the adipose tissue. We propose that distinct lipid-buffering adipose tissue macrophages rely on metabolites, which shape their epigenetic landscape. We contribute to the SFB by providing the consortium with a variety of fate mapping and reporter mouse lines (T. Weichhart, M. Schweiger, E. Pohl, G. Egger, A. Haschemi, C. Moissl-Eichinger). We will generate macrophage data from the adipose tissue in different models of obesity and their associated analyses (E. Pohl, G. Egger, A. Haschemi). Furthermore, we will provide the samples from obese and respective lean mice for the analyses of the microbiome. Differences in the microbiota concerning “healthy obese” versus “sick obese” may influence various aspects of adipose tissue function (C. Moissl-Eichinger). Crosstalk of murine and human adipose tissue macrophages with the stromal vascular fraction will be analyzed in co-culture models and 3D-organoids (M. Schweiger). Together with members of the consortium we will decipher the contribution of metabolic pathways such as one carbon metabolism, fatty acid oxidation and TCA cycle to epigenetic programming of beneficial adipose tissue macrophages (A. Haschemi, T. Weichhart, M. Schweiger, E. Pohl, G. Egger).
Medical University of Vienna
Center for Physiology and Pharmacology
Institute for Vascular Biology and Thrombosis Research